Sampath Malathi, Pichaimani Arunkumar, Kumpati Premkumar and Sengottuvelan Balasubramanian
Laboratory of Bioorganic Chemistry, Department of Biotechnology, Indian Institute of Technology Madras, Chennai, India
Breast cancer is one of the leading causes of death for cancer in human female patients, characterized by a high degree of recurrence after surgical removal and first-line treatment with chemotherapeutics. Many chemotherapeutic drugs such as taxol, cisplatin, doxorubicin, 5-flurouracil, camthothecin and metroaidazole have been used in clinical practice. Chemotherapeutic drugs are highly cytotoxic and typically large doses of chemotherapeutic drugs are needed to produce optimal therapeutic response. To overcome these side effects, curcumin, which is a naturally occurring compound with potent anti-cancer activity, has been used clinically. Curcumin, a polyphenolic compound derived from dietary spice turmeric, possesses diverse pharmacological effects including anti-inflammatory, antioxidant, antiproliferative and antiangiogenic activities. Phase i clinical trials have shown that curcumin is safe even at high doses (12 g/day) in humans but exhibit poor bioavailability due to its lack of solubility in aqueous solvents. The bioavailability of curcumin has been increased by poly (lactic-co-glycolic) acid [plga (60/40)] nanoparticles with different capping agents (chitosan, peg and dextran) and emulsifier (tocopherol poly(ethylene glycol)1000 succinate: tpgs) with good drug loading and delivery performance. Plga based nanoparticles were prepared by emulsion solvent evaporation method and were characterized. The encapsulation efficiency of curcumin by plga nps with different capping agents and emulsifier lies in the range of 82% to 89% and the anti-oxidant activity is 80%. In vitro anticancer activity of curcumin loaded plga nanoparticles with different capping agents on mcf- 7 indicates that they are more effective in arresting cell growth. The cellular uptake of tpgs emulsified dextran capped curcumin encapsulated plga nps is much higher when compared to that of plga nps with other capping agents, emulsifier and free curcumin.
Keywords: Antioxidant Activity, Breast Cancer, Cytotoxicity, Dextran, Poly (Lactic-Co-Glycolic) Acid, Tpgs Emulsifier.
 S. Malathi, L. Rachita, K. Purnasai and S. Balasubramanian, Colloids Surf B, 2014, 117, 128.
 P. Anand, H. B. Nair, B. Sung, A. B. Kunnumakkara, V. R. Yadav R. R. Tekmal and B. B. Aggarwal, Biochem. Pharmacol, 2010, 79, 330.
 A. Mukerjee and J. K. Vishwanathaa, Anticancer Res, 2009, 29, 3867.
 P. Ananthakrishnan, F. L. Balci and J. P. Crowe, Int. J. Surg. Oncol, 2012, 2012, 585.
 F. Danhier, E. Ansorena, J. M. Silva, R. Coco, A. Lebreton and V. Preat, J. controlled Release, 2012, 161, 505.