Mohammad Yasin Mohammad, M. Iqbal Choudhary and Atta-ur-Rahman
Department of Pharmacy, Faculty of Pharmacy, Middle East University, Amman-11831, Jordan
Microbial transformation of thymoquinone , by suspended cell-cultures of the plant pathogenic fungus Aspergillus niger, resulted in the production of three metabolites. These metabolites were identified as 5-isopropyl-2-methyl-2,4-cyclohexenone lactone , hydroxythymoquinone  and 4-hydroxy-2-isopropyl-5-methylphenol , by different spectroscopic methods. Metabolite 2 was found to be new. Compound 4 showed potent antioxidant activity.
Keywords: Thymoquinone, Aspergillus niger, fungal transformation, oxidation, reduction, antioxidant activity.